24-hour urine protein quantitative

Sulfosalicylic acid method
This is the method commonly used by the hospital laboratory

Reagent preparation
Preparation of sulfosalicylic acid 3 g, add distilled water to 100 ml into 3% sulfosalicylic acid solution. Store the perfect solution in a brown bottle and put them in the sunless dark to save, in order to avoid failure.

Test method
Take a clean glass test tube and add 2 ml of urine. Then use the drop dropping reagent 3 ~ 5 drops in urine to observe whether there is protein. According to the turbidity, sediment and solidification degree, determine the "+" (plus) number.

From+-±, judgment standard
“-”: A minus sign is negative, indicating no protein. Adding reagent, urine is still clearly. Even in a black background you still do not see the turbid
performance.
"±": a plus and a minus sign represent trace protein in urine. After adding reagent appears white mild cloudy, but it can only be seen in the black background.
"+": A plus represents medium weight protein in the urine. After joining the reagents, white light cloudy appears without floc particles.

"++": 2 plus represents there is protein in the urine. After joining the reagents, noticeable white precipitate appears.
"+ + +":3 plus represents there is large quantity of protein in urine. After joining the reagents, flocculent white precipitate appears.
"+ + + +":4 plus represents there are a large number of protein in urine. After adding reagent, solidified into a block appear immediately, like jelly.
According to turbidity reaction, estimates the urine protein content.

As follows: Urine protein <0.1g/L: -
Urinary protein is 0.1 - 0.2g/L: ±
Urinary protein is 0.2 - 1.0g/L: +
Urinary protein is 1 ~ 2.0g/L: ++
Urinary protein is 2 - 4.0g/L: + + +
Urine protein >4.0g/L: + + + +

Heating method
The method is simple. It does not need any reagent.
First, take a glass test tube, pour the urine to be checked at two-thirds and add 2% acid or a few drops of vinegar.

Second, thumb and index finger take the bottom of the tube inclined and put it on the flame (such as alcohol lamp) directly to heat tube at the upper end of the urine. Turn the tube frequently until the urine in the upper tube boiling.

Third, observe whether there is turbidity or precipitation coagulation. In order to eliminate the turbid is not false positive caused by proteins, you should add 2 to 3 drops of acetic acid or 10 more or less drops of vinegar. If this opacity not disappears, it is positive response, which shows that it is protein.

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